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Fatty Acid Analysis

Understand Lipid Profiles with Precision

From routine fatty acid profiling to custom analyses across food, feed, and supplements, our fatty acid testing services support regulatory compliance, product development, and research.

What We Test:

  • Oils and fats (edible and industrial)

  • Supplements (fish oil, algal oil, flaxseed, etc.)

  • Animal feeds and pet foods

  • Food products (meat, dairy, eggs, etc.)

  • Biological samples (blood, tissue extracts, milk)

Why It Matters:

  • Nutritional labelling & regulatory compliance

  • Research & product development

  • Shelf life, oxidation, and formulation optimization

Common Fatty Acid Tests

Lipid Labs provides comprehensive fatty acid profiling to support nutrition labeling, product development, regulatory compliance, and research. Our analyses cover a wide range of matrices, including oils, supplements, animal feeds, food products, and biological samples.

Fatty Acid Profile

Our Total Fatty Acid Profiling includes comprehensive quantification of:​

  • Saturated Fatty Acids (SFAs): Assessing levels of SFAs to aid in nutritional evaluations.​

  • Monounsaturated Fatty Acids (MUFAs): Determining MUFA content for health-related product claims.​

  • Polyunsaturated Fatty Acids (PUFAs): Including essential fatty acids such as Omega-3 and Omega-6, crucial for various bodily functions.​

    • Omega-3 Fatty Acids: Including EPA, DHA, and ALA, important for cardiovascular and cognitive health.​

    • Omega-6 Fatty Acids: Such as linoleic acid (LA), vital for growth and development.​

This analysis provides a detailed breakdown of fatty acid composition, supporting accurate labeling and health claims.

Need a Custom Test Panel?

We collaborate with clients to develop custom fatty acid analysis packages tailored to specific product or research needs.

Contact us

Additional Specialized Analyses

  • Trans Fatty Acid Content: Detection and quantification of trans fats in various matrices to ensure compliance with health regulations.​

  • Medium-Chain Fatty Acids (MCFAs): Quantification of C6–C12 chain fatty acids, commonly found in coconut and palm kernel oils, relevant for energy metabolism studies.​

  • Short-Chain Fatty Acids (SCFAs): Analysis of butyric, acetic, and propionic acids, significant in gut health and metabolic research.​

  • Conjugated Linoleic Acid (CLA): Quantification of CLA isomers pertinent to dairy, ruminant meats, and supplement products, associated with various health benefits.

Test Methods

We primarily employ Gas Chromatography with Flame Ionization Detection (GC-FID) for fatty acid quantification due to its precision and reliability. For complex matrices or specialized applications, Gas Chromatography–Mass Spectrometry (GC-MS) is available upon request.​

All methods adhere to ISO/IEC 17025 standards, ensuring high-quality and consistent results.

Sample Requirements

  • Sample Types: Oil, powder, capsule, food matrix, or biological fluids.​

  • Minimum Sample Volume: 1–2 g or mL.​

  • Packaging: Secure, leak-proof containers.​

  • Turnaround Time: Typically 5–10 business days.

Submit a sample: Submission Form

Sterol and Oxysterol Testing

Sterols and oxysterols are critical components in many biological and commercial products, playing key roles in cholesterol metabolism, membrane function, oxidative stability, and health-related claims. At Lipid Labs, we offer specialized sterol and oxysterol testing to support research, product development, and regulatory compliance.

Sterols

  • Phytosterol Analysis
    Quantification of common plant sterols including beta-sitosterol, campesterol, and stigmasterol. Useful for functional food and supplement claims.
  • Cholesterol Content
    Measurement of cholesterol in oils, dairy, meat, and supplements. Supports nutritional labelling and formulation validation.
  • Sterol Composition Profiles
    Full profiling of sterols in plant and animal-derived lipids for characterization and regulatory documentation.

​This analysis provides a detailed breakdown of fatty acid composition, supporting accurate labeling and health claims.

Oxysterols

Common Oxysterol Targets

  • 7-ketocholesterol
  • 7beta-hydroxycholesterol
  • 24S-hydroxycholesterol
  • 25-hydroxycholesterol
  • 27-hydroxycholesterol

These oxidized cholesterol derivatives are measured in a range of matrices to support disease research, quality control, and lipid oxidation studies.

Test Methods

We use Gas Chromatography (GC) and Gas Chromatography–Mass Spectrometry (GC-MS) for sterol and oxysterol testing, depending on the required sensitivity and specificity. Methods are validated and compliant with ISO/IEC 17025 standards.

Sample preparation includes lipid extraction, derivatization, and selective detection using internal standards.

Sample Requirements

  • Sample Types: Oil, powder, capsule, food matrix, or biological fluids.​

  • Minimum Sample Volume: 1–2 g or mL.​

  • Packaging: Secure, leak-proof containers.​

  • Turnaround Time: Typically 5–10 business days.

Submit a sample: Submission Form

Custom Panels and Research Support

We offer custom sterol and oxysterol panels for research and commercial applications. Whether you’re developing a functional food, validating a health claim, or exploring cholesterol metabolism in clinical studies, our team can support your analytical needs.

Fat-Soluble Vitamin Testing

At Lipid Labs, we provide targeted analysis of fat-soluble vitamins to support regulatory compliance, nutritional research, and product formulation. Our methods are optimized for accurate quantification of tocopherols and tocotrienols in oils, supplements, and biological matrices.

Vitamin E

This analysis provides a detailed breakdown of fatty acid composition, supporting accurate labeling and health claims.

Tocopherols

Common Oxysterol Targets

  • 7-ketocholesterol
  • 7beta-hydroxycholesterol
  • 24S-hydroxycholesterol
  • 25-hydroxycholesterol
  • 27-hydroxycholesterol

These oxidized cholesterol derivatives are measured in a range of matrices to support disease research, quality control, and lipid oxidation studies.

Tocotrienols

  • Alpha-Tocotrienol
  • Beta-Tocotrienol
  • Gamma-Tocotrienol
  • Delta-Tocotrienol

Test Methods

Vitamin E compounds are measured using High-Performance Liquid Chromatography (HPLC) with fluorescence or UV detection, depending on matrix and application. All methods are validated under ISO/IEC 17025 standards.

Sample preparation includes lipid extraction and cleanup for improved specificity and recovery.

Sample Requirements

  • Sample Types: Oil, powder, capsule, food matrix, or biological fluids.​

  • Minimum Sample Volume: 1–2 g or mL.​

  • Packaging: Secure, leak-proof containers.​

  • Turnaround Time: Typically 5–10 business days.

Submit a sample: Submission Form

Additional Tests

In select matrices, we also offer analysis for:

  • Vitamin A (Retinol, Retinyl Esters)
  • Vitamin D (Cholecalciferol)

Contact us to confirm method suitability for your product or research needs.

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